Abazied, M., El-Damarany, H., Sadek, F., Mohamed, A. (2025). Cytotoxicity Evaluation and Phytochemical Investigation of n-hexane extract of Centaurea glomerata Vahl. Aswan University Journal of Environmental Studies, 6(2), 164-182. doi: 10.21608/aujes.2025.368400.1336
Mostafa M. Abazied; Hussein A. El-Damarany; Fatma H. Sadek; Abou El-Hamd H. Mohamed. "Cytotoxicity Evaluation and Phytochemical Investigation of n-hexane extract of Centaurea glomerata Vahl". Aswan University Journal of Environmental Studies, 6, 2, 2025, 164-182. doi: 10.21608/aujes.2025.368400.1336
Abazied, M., El-Damarany, H., Sadek, F., Mohamed, A. (2025). 'Cytotoxicity Evaluation and Phytochemical Investigation of n-hexane extract of Centaurea glomerata Vahl', Aswan University Journal of Environmental Studies, 6(2), pp. 164-182. doi: 10.21608/aujes.2025.368400.1336
Abazied, M., El-Damarany, H., Sadek, F., Mohamed, A. Cytotoxicity Evaluation and Phytochemical Investigation of n-hexane extract of Centaurea glomerata Vahl. Aswan University Journal of Environmental Studies, 2025; 6(2): 164-182. doi: 10.21608/aujes.2025.368400.1336
Cytotoxicity Evaluation and Phytochemical Investigation of n-hexane extract of Centaurea glomerata Vahl
1Department of Chemistry, Faculty of Science, Aswan University, Egypt
2Chemistry Department, Faculty of Science, Aswan University, Egypt
Abstract
GenusCentaurea, known for its diverse pharmacological properties, has been extensively studied for its essential oils (EOs) and bioactive compounds. This study investigated the dichloromethane: methanol (CH2Cl2: CH3OH) (1:1 v/v) extract and the eluted n-hexane fraction of Centaurea glomerata, focusing on its volatile components and cytotoxic potential against Hepatocellular carcinoma (HepG2) and Breast adenocarcinoma (MCF-7) cell lines. Moreover, the study included isolation of three interested compounds. Cytotoxicity assays revealed that the methanol: dichloromethane (CH2Cl2:CH3OH) (1:1 v/v) extract exhibited significant growth inhibition in both cancer cell lines at higher concentration of 100 µg/ml, while the isolated compounds showed limited activity against cancer cells. These findings suggested that the crude extract of dichloromethane: methanol of Centaurea glomerata exhibits synergistic effect, which is the interaction of two or more constituents when their combined effect is greater than the sum of the effects seen when each constituent is given alone. This effect enhances the extract efficacy against diverse cancer cells.
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