Ali, M., Mahmoud, S., abbas, M., ahmed, A. (2023). Antibiotic-tolerant Rhizobium leguminosarum biovar.vicia in Egyptian Soil. Aswan University Journal of Environmental Studies, 4(4), 237-247. doi: 10.21608/aujes.2023.210693.1152
Mariem Ali; Salah Mahmoud; mohamed tawfik abbas; Alshaymaa ahmed. "Antibiotic-tolerant Rhizobium leguminosarum biovar.vicia in Egyptian Soil". Aswan University Journal of Environmental Studies, 4, 4, 2023, 237-247. doi: 10.21608/aujes.2023.210693.1152
Ali, M., Mahmoud, S., abbas, M., ahmed, A. (2023). 'Antibiotic-tolerant Rhizobium leguminosarum biovar.vicia in Egyptian Soil', Aswan University Journal of Environmental Studies, 4(4), pp. 237-247. doi: 10.21608/aujes.2023.210693.1152
Ali, M., Mahmoud, S., abbas, M., ahmed, A. Antibiotic-tolerant Rhizobium leguminosarum biovar.vicia in Egyptian Soil. Aswan University Journal of Environmental Studies, 2023; 4(4): 237-247. doi: 10.21608/aujes.2023.210693.1152
Antibiotic-tolerant Rhizobium leguminosarum biovar.vicia in Egyptian Soil
1Department of Agricultural Microbiology, Faculty of Agriculture and Natural Resources, Aswan University, Egypt
2Department of soil Microbiology, Faculty of Agriculture, Assiut University, Egypt
3Department of Agricultural microbiology, Faculty of Agriculture & Natural Resources, Aswan university
4Department of Agricultural Microbiology, Faculty of Agriculture, Beni-Suef University, Beni-Suef 18116, Egypt
Abstract
Extensive use of antibiotics and their mismanagement significantly contribute to the emergence of soil microorganisms that are resistant to antibiotics, especially in the root zone. In this study, twenty-one isolates of Rhizobium leguminosarum biovar vicia were isolated from three cultivars of faba- bean that is cultivated in the El Shenab area of Aswan Governorate. For these isolates, morphological, cultural, and biochemical characteristics were examined. 21 isolates of the soil bacterium Rhizobium leguminosarum were screened for their response to three widely used antibiotics by using the minimum inhibitory concentration (MIC) test. The isolates were grown in a yeast mannitol ager (YMA) medium, which contains different concentrations (100 – 1000 PPM) of three antibiotics commonly used in Egypt (amoxicillin, cefotaxime, and tetracycline) individually. Six resistant isolates were obtained. The isolates (40A, 40B, and 843B) were the most resistant of the six isolates to antibiotics with high efficiency under the concentration of 1000 PPM for the three antibiotics. The percentage of tolerance and inhibition of the antibiotic for these three isolates was Amoxicillin (72%, 65%, and 45%), Cefotaxime (60%, 55%, and 47%), and Tetracycline (65%, 55%, and 51%) respectively. The three isolates showed high efficiency in breaking down antibiotics. These isolates can be used in agricultural farms that contain antibiotics.
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